MagiCyte MB

Culture Methods

Take Salmonella testing in food as an example. Culture methods, especially reference methods such as FDA BAM, USDA MLG and ISO, are widely used for Salmonella detection and isolation. Unlike detection-only molecular methods, culture methods are indispensable for obtaining discrete Salmonella colonies, which are needed by numerous downstream confirmation and characterization technologies.

Reference Methods

Reference methods for Salmonella detection and isolation usually include multiple time-consuming and resource-intensive culture steps. For example, FDA BAM requires three culture steps with each step taking about one day: first in non-selective enrichment media, then in selective enrichment broths, and finally in selective agars. As a result, labs have to wait until Day 4 to get detection results and obtain colonies before they can start downstream analyses.

PCR

With molecular methods such as PCR, labs can get detection results faster. But for presumptive positive samples, labs need isolates for downstream analyses and still have to wait until Day 4 due to the subsequent use of the FDA BAM method.

MagiCyte MB

In MagiCyte MB, Salmonella are first separated from the samples (which might contain competing microbiota, chemical, inhibitors, etc.) by magnetic beads coated with anti-Salmonella antibodies through a selective enrichment step called immunomagnetic separation (IMS). The separated Salmonella are then plated into selective agar where the microcolonies' morphology are analyzed in real time for rapid and reliable detection and enumeration. Top-ranked discrete colonies are automatically picked and placed in an "output" plate for downstream analyses.

FASTER

Salmonella doubling time is less than 30 minutes when incubated at 37 °C, i.e., in a few hours, their colonies are easily visible under a low-resolution microscope and contain enough cells for downstream analyses. Why wait 4 days? MagiCyte obtains colonies days earlier and allows downstream tests to start much sooner.

SMALLER

Why use Petri dishes for microbial culture? By replacing Petri dishes with a standard 96-well plate, MagiCyte MB significantly saves space and materials.

EASIER

A true walk away solution: samples in, results & colonies out.

Screening? Enumeration? Isolation? Just select the mode on the intuitive software user interface and MagiCyte MB will do it for you.

BETTER

How are the colonies formed? Are the colonies discrete? Real time colony analysis by AI provides the answers to achieve accurate detection, enumeration and colony picking. Automation and standardization make the process reliable and repeatable.